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Position: Home > Faculty & staff > Faculty-M.Sc Supervisor > Content     



Name:Zhang Cunzheng


Address:Zhongling St.50, Xuanwu strict, Nanjing210014, China




B.Sc. in Plant Protection, Chemistry, entomology 1993-1997.Department of Plant Protection, Nanjing Agricultural University, Nanjing, China, Field of study: entomology/chemistry. Advisor: Prof. Jinliang, Shen

Ph.D. in Insect toxicology and Molecular Biology 2004, 9 – 2011, 6: Laboratory of Insect Biochemistry and Molecular Biology, Department of Plant Protection, Nanjing Agricultural University, China. Field of study: entomology/toxicology. Advisor: Prof. Yidong, Wu

Concentrations: Recombinant antibody Library development via phase-display technique for the multi-drug residues detection and Insect toxicology mechanism study; Agrochemical Ecotoxicology and Health crisis evaluation; Environmental fate; Mechanism research of cumulative side-effect of multi-agrochemical residues for the model animal health

International Joint Ph.D. Student program of MOE (China)-AAFC (Canada), in 2007, 9-2008, 9: Food Research & Development Center of Agriculture and Agricultural Food of Canada, Canada; Field of study: Biochemistry. Advisor: Dr. Deschênes, Louise

Concentrations: Block Copolymer and Protein Nano-patterning film for the biosensor and biomaterials application

Professional Experience

08/1997/8-08/2000. Faculty: Institute of Plant protection of Jiangsu Academy of agricultural Sciences. Nanjing, China

09/2000-09/2006. Assistant professor: Institute of Food safety of Jangsu Academy of agricultural sciences, Nanjing, China

08/2004-12/2006. Adjunct lecture: Jinling Institute of Technology. Nanjing, China

09/2006-09/2007. Researcher fellow, National Food Safety and Toxicology center, Michigan State University, USA.

10/2007-present. Professor: Institute of Food safety and nutrition of Jangsu Academy of agricultural sciences. Nanjing, China

08/2014-present. Adjunct professor: Department of Plant Protection, Nanjing Agricultural University, Nanjing, China

08/2013-present. Adjunct professor: School of food science & technology, Nanchang University, Nanchang, China.

08/2013-present. Adjunct professor: School of food & drug, Anhui Science & technology University, Benbu, China.

08/2018-present. Adjunct professor: School of food and biological engineering, Jiangsu University, Zhenjiang, China.

05/2018-present. Expert of pest and pesticide management of UN-FAO, Rome, Italy.

Scientific Research Field

Food safety and nutrition, pesticide residue, plant protection and environmental toxicology, food fraud, foodborne pathogenic microorganism, risk assessment,

Social academic post and honor

Membership of Royal Society of Chemistry (RSC, UK, Membership ID: 549136), number of expert (think) tank for MOST and Department of Science & Technology of Jiangsu Province (2016-present), number of expert(think) tank of the Food Science and Engineering Professional Cooperation Committee of General Undergraduate Colleges of Anhui Province(2017-present), China. Member of council of innovative and strategic alliance of detection technique for food-born microorganisms, China (2017-present).

Teaching Courses

Published papers

1.Sa Dong, Yuan Liu, Xiao Zhang, Chongxin Xu, Xianjin Liu, Cunzheng Zhang*. Development of an immunochromatographic assay for the specific detection of Bacillus thuringiensis (Bt) Cry1Ab toxin. Anal Biochem. 2018 Aug 18. pii: S0003-2697(18)30301-4. DOI: 10.1016/j.ab.2018.08.014

2.Yulou Qiu, Pan Li, Sa Dong, Xiaoshuai Zhang, Qianru Yang, Yulong Wang, Jing Ge, Bruce D. Hammock, Cunzheng Zhang*, Xianjin Liu, Phage-Mediated Competitive Chemiluminescent Immunoassay for Detecting Cry1Ab Toxin by Using an Anti-Idiotypic Camel Nanobody, Journal of Agricultural and Food Chemistry, 2018, 66(4): 950-956

3.Dong, Sa# ; Zhang, Cunzheng#; Liu, Yuan; Zhang, Xiao; Xie, Yajing; Zhong, Jianfeng; Xu, Chongxin; Liu, Xianjin, Simultaneous production of monoclonal antibodies against Bacillus thuringiensis (Bt) Cry1 toxins using a mixture immunization , ANALYTICAL BIOCHEMISTRY, 2017.8.15, 531: 60~66.

4.Beibei Liu; Hang Gong; Yulong Wang; Xiaoshuai Zhang; Pan Li; Yulong Qiu; Liming Wang; Xiude Hua; Yirong Guo; Minghua Wang; Fengquan Liu; Xianjin Liu; Cunzhang Zhang* A gold immunochromatographic assay for simultaneous detection of parathion and triazophos in agricultural products, Analytical Methods, 2017.12.11, 10: 422~428

5.Xu, Chongxin ; Zhang, Cunzheng ; Zhong, Jianfeng; Hu, Hui; Luo, Shimin; Liu, Xiaoqin; Zhang, Xiao; Liu, Yuan; Liu, Xianjin. Construction of an Immunized Rabbit Phage Display Library for Selecting High Activity against Bacillus thuringiensis Cryl F Toxin Single-Chain Antibodies. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017.7.26, 65(29): 6016~6022

6.Chongxin Xu, Xiaoqin Liu, Cunzheng Zhang, Xiao Zhang, Jianfeng Zhong, Yuan Liu, Xiaodan Hu, Manman Lin, Xianjin Liu*. Establishment of a sensitive time-resolved fluoroimmunoassay for detection of Bacillus thuringiensis Cry1Ie toxin based nanobody from a phage display library.Analytical Biochemistry,2017,(518):53-59.

7.Tan Liu, Cunzheng Zhang, Jing Peng, Zhiyong Zhang, Xing Sun, Hui Xiao, Ke Sun, Leiqing Pan, Xianjin Liu, Kang Tu. Residual Behaviors of Six Pesticides in Shiitake from Cultivation to Postharvest Drying Process and Risk Assessment. J. Agric. Food Chem. 2016, 30; 64(47):8977-8985.

8.Sa Dong, Cunzheng Zhang, Xiao Zhang,Yuan Liu, Jianfeng Zhong, Yajing Xie,Chongxin Xu,Ying Ding,Liuquan Zhang,Xianjin Liu*.Production and Characterization of Monoclonal Antibody BroadlyRecognizing Cry1 Toxins by Use of Designed Polypeptide as Hapten[J]. Analytical Chemistry, 2016, (88):7023-7032.

9.Cunzheng Zhang, Li Wang, Zhui Tu, Xing Sun, Qinghua He, Zhaojing Lei, Chongxin Xu,Yuan Liu, Xiao Zhang, Jingyi Yang, Xianjin Liu, Yang Xu. Organophosphorus pesticides Detection using Broad specific Single-Stranded DNA based Fluorescence Polarization Aptamer Assay. Biosensors and Bioelectronics, 55(2014), 216–219.

10.Cun-Zheng Zhang, Zhi-Yong Zhang, Xian-Jin Liu,Wayne Jiang, Yi-Dong Wu. Dissipation and environmental fate of herbicide H-9201 in carrot plantings under field conditions, Food Chemistry, 119 (2010): 874–879.

11.ZHANG Cun-Zheng, YANG Chun-Long, LIU Xian-Jin, CHEN Min, YU Jie. An Indirect Competitive Enzyme-Linked Immunosorbent Assay for Determination of New Herbicide H-9201 in Water. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2011, 39(23), 225–230.

12.ZHANG Cun-zheng, ZHANG Xin-ming, TIAN Zi-hua, He Dan-jun, LIU Xian-jin. Degradation of Chlorpyrifos and Fipronil in Rice from Farm to Fork and Risk Assessment. Agricultural Sciences in China, 2010, 9(5): 754-763.

13.Xiao Zhang,Cunzheng Zhang,Yuan Liu,Xiangyang Yu,Xianjin Liu. Construction of scFv phage display library with hapten-specific repertories and characterization of anti-ivermectin fragment isolated from the library. Eur Food Res Technol, 2010, 231:423-430.

14.Xiao Zhang、Chongxin Xu、Cunzheng Zhang、Yuan Liu、Yajing Xie、Xianjin Liu. Established a new double antibodies sandwich enzyme-linked immunosorbent assay for detecting Bacillus thuringiensis (Bt) Cry1Ab toxin based single-chain variable fragments from a naïve mouse phage displayed library. Toxicon, 2014,81,13-22.

15.Qing-hua He, Yang Xu, Cun-zheng Zhang, Yan-ping Li, Zhi-bing Huang. Phage-borne peptidomimetics as immunochemical reagent in dot-immunoassay for mycotoxin zearalenone. Food Control, Volume 39, May, Pages 56–61. 2014.

16.Li Min, Zhu Min, Zhang Cunzheng, Liu Xianjin, Wan Yakun. Uniform orientation of biotinylated nanobody as an affinity binder for detection of Bacillus thuringiensis (Bt) Cry1Ac toxin. Toxins (Basel). 2014,6(12):3208-22.

17.Xiao Zhang, Yuan Liu, Cunzheng Zhang, Yun Wang, Chongxin Xu, XianjinLiu.. Rapid isolation of single-chain antibodies from a human synthetic phage display library for detection of Bacillus thuringiensis (Bt) Cry1B toxin. Ecotoxicology and Environmental Safety, 2012,81 (1):84–90.

18.Wang, Y; Zhang, X; Zhang, CZ; Liu, Y; Liu, XJ. Isolation of single chain variable fragment (scFv) specific for Cry1C toxin from human single fold scFv libraries. Toxicon,2012; 60 (7): 1290-1297.

19.Pingyan Wang,Guanghui Li, Junrong Yan, Yonghong Hu, Cunzheng Zhang, Xianjin Liu, Yakun Wan. Bactrian camel nanobody-based immunoassay for specific and sensitive detection of Cry1Fa toxin. Toxicon. 2014,15;92:186-92.

20.Wang Li, Liu Xianjin, Zhang Qiang, Zhang Cunzheng, Liu Yuan, Tu Kang ,Tu Jian. Selection of DNA aptamers that bind to four organophosphorus pesticides. Biotechnology Letters,2012,34(5):869-874

21.Chongxin Xu, Xiao Zhang, Xiaoqin Liu, Yuan Liu, Xiaodan Hu, Jianfeng Zhong, Cunzheng Zhang, Xianjin Liu*. Selection and application of broad-specificity human domain antibody for simultaneous detection of Bt Cry toxins [J].Analytical Biochemistry,2016,(512):70-77.

22.Yuan Liu, Aihua Wua, Jing Hu, Manman Lin, Mengtang Wen, Xiao Zhang,Chongxin Xu,Xiaodan Hu,Jianfeng Zhong, Lingxia Jiao,Yajing Xie,Cunzhen Zhang,Xiangyang Yu,Ying Liang,Xianjin Liu. Detection of 3-phenoxybenzoic acid in river water with a colloidal gold-based lateral flow immunoassay [J].Analytical Biochemistry, 2015, 483, 7-11.

The other articles will be provided with requirement.

Main Scientific Research Projects

IR-4 project (USDA, USA) and Agriculture and Agriculture food Canada (AAFC)-Food research and development center (AAC) project for pesticide residue detection and food safety related biosensor development,

the State's Key Project of Research and Development plan at the Program of reducing the chemical pesticide and chemical fertilizer application at crops” (Project No. 2016YFD0200803-3) for food system sustainability and food safety and quality, focus on risk assessment of pesticide and biodiversity investigation of soil,

the State's Key Project of Research and Development plan of China at “Food safety technology development and food chain management for supermarket scenario”(Project No. 2006BAK02A28).


Scientific Research Achievements and Awards

2004:  First Class Prizes of Liyang government Science and Technology Award

2004:  Second Class Prizes of Changzhou government Science and Technology Award

2014:  Second Class Prizes of Jiangsu Province government Science and Technology Award

2014:  Third Class Prizes of Nanjing government Science and Technology Award

2015:  First Class Prizes of Jiangsu Academy of agricultural Sciences Science and Technology Award

2016:  First Class Prizes of China Society of Plant Protection Science and Technology Award

2017:  First Class Prizes of Ministry of agriculture of China Agricultural Science and Technology Award

2017:  First Class Prizes of Jiangsu Province government Science and Technology Award

Number of postgraduates under supervision


Number of supervised masters and Ph.D.


Number of supervised undergraduates


The above information updated


Returns〗     Hits:486   Date:2019-4-3

Returns〗   Hits:   Date:2019-04-03  
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