Chinese  |    Jiangsu University      

Ph.D program of Agricultural Product Processing and Storage Engineering was acquired first by Jiangsu University in China……[More]

    Apply Now!
The graduate programs in our school are recruiting overseas studentsr, please apply as soon as possible.
    Quick Links
Jiangsu University
Graduate Department of JU
International Office of JU
Overseas Education College of JU
Position: Home > Young Teacher Positions > Content     
Integration of transcriptome and proteome data reveals Ochratoxin A biosynthesis regulated by pH in Penicillium citrinum

Ochratoxin A (OTA) has been found in a wide range of commodities and it is highly toxicity to both humans and animals. Therefore, a good understanding of the mechanisms of OTA production by fungi will contribute to the development of eco-friendly methods to mitigate the toxin. In this study, the results showed that Penicillium citrinum X9-4, which was isolated from infected grapes by our laboratory, produced the highest amount of OTA at pH 5 in culture media, and toxin-production was restrained under acidic environment (pH 3). And then differentially expressed proteins of P. citrinum X9-4 cultured under these two conditions were analyzed by proteomic technology. Additionally, through the analysis of the transcriptome data of P. citrinum cultured at pH 3 and 5, the differentially expressed genes were found to be involved in many metabolic pathways, including "amino acid transport and metabolism", "transport and metabolism of carbohydrates", "inorganic ion transport and metabolism", "biosynthesis of secondary metabolites" and "energy and supply metabolism", which are likely to be involved in regulating OTA biosynthesis. It also revealed that the expression levels of some OTA synthesis related enzymes and genes for regulating toxin synthesis pathways were reduced under acidic culture condition, such as acetyltransferase, acyl coenzyme A oxidase, alcohol oxidase, cytochrome P450, acetyl xylan esterase, turn ketol enzyme and so on.


Fig. 1 Two-dimensional pattern of total proteins of P. citrinum cultured at different pH. a: P. citrinum cultured at pH 3, b: P. citrinum cultured at pH 5.

Fig. 2 Functional classification of DEG in P. citrinum. A: GO classification. B: COG classifications.

Returns〗 Hits: Date:2017-10-25
About Our School Undergraduate Program Graduate Program Research Faculty & staff
  A survey
List of undergraduate program
Teaching resources
A survey
Introduction of graduate program
List of Graduate Program
Major research fields
Centers or Institutes of research
Relevant achievements and subject characteristics
An introduction
List of staff
Faculty-PhD. Supervisor
Faculty-MA. Supervisor
Copyright © 2015-2019 Jiangsu University - School of Food and Biological Engineering All Right
Add: School of Food and Biological Engineering, Jiangsu University,301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China
Tel: 0086-511-88780201 Fax: 0086-511-88780201 E-mail:   Powered by