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讲准字149号:Impact of individual and mixtures of mycotoxins and persistent organochloride pesticides on reproduction - in vitro investigations

题目:Impact of individual and mixtures of mycotoxins and persistent organochloride pesticides on reproduction - in vitro investigations

主讲:YUN YUN GONG

时间:530 900

地点:文华楼506会议室

主办:食品与生物工程学院

主讲简介:YUN YUN GONG2000年毕业于中国预防医科院,获博士学位,现为英国利兹(Leeds)大学食品科学与营养学院教授,2017年受聘我校兼职教授。宫博士长期从事食品卫生的教学和科研工作,研究方向主要为食品霉菌毒素的安全性评价和人群预防措施。近年来先后独立承担英国皇家协会,维康基金会,美国国家环境科学研究院(NIEHS), WHO肿瘤研究机构(IARC),其它慈善基金会的多项课题,致力于儿童人群霉菌毒素暴露水平和健康效应评价和健康效果的机制研究和干预。宫博士与世界知名研究机构如Johns Hopkins大学,伦敦热带病医学研究所(LSHTM)IARC,南非医学研究署有多项合作项目,目前是利兹大学食品霉菌毒素科研组的带头人。作为学科领域的学术带头人,先后承担国际研究项目7项在国际该领域杂志上发表论文近40,参与著书4册,被多次邀请参加国际学术会议做报告,应邀在康奈尔大学,比尔盖茨基金会专家研讨会上做重要学术讲座,目前是世界霉菌毒素研究学会成员;英国环境突变学会成员;英国肿瘤研究学会会员,中国食品毒理学会会员。


主讲内容:

Environmental and food-borne toxins e.g. mycotoxins and persistent organochloride pesticides (POPs) have been suggested to have endocrine disrupting effects. However, the reproductive toxicological effect of both individual and mixtures of these toxins are little understood. This research explores various in vitro cellular models, namely the reporter gene assay (RGA) using MMV-Luc, cytotoxicity and hormonal alteration in MA-10 Leydig cell line and BeWo placental cell lines to evaluate the toxicological effects on reproduction by individual and mixtures of selected mycotoxins zearalenone (ZEN), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), deoxynivalenol (DON) and ochratoxin A (OTA) and POPs 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (DDT) and its metabolite 1, 1, 1-trichloro-2,2-bis (p-chlorophenyl)-ethane (DDE). In the RGA study, ZEN and α- ZOL at biologically relevant concentrations induced oestrogen receptor (ER) transcriptional response (75% - 85%) similar to the response mediated by 10 nM of 17β-oestradiol (E2). Co-exposure of single mycotoxins with clinically relevant concentrations of E2 (0.05 nM or 10 nM) had stimulatory or inhibitory effect on E2-mediated ER transcriptional response depending on dose whereas mixtures of equimolar concentration (50 µM) of DDT and DDE with E2 at similar doses completely abolished ER response. Among single toxins, DON and OTA were significantly cytotoxic to both MA-10 and BeWo cell lines. In the endocrine function, ZEN and its metabolites elevated progesterone production, but inhibited testosterone level in MA-10 cells whereas they had no effect on progesterone levels in BeWo cells, but significantly increased E2 production. All the tested mycotoxins and POPs significantly inhibited the production of beta-human chorionic gonadotropin (β-hCG) in BeWo placental cells. The effects of binary mycotoxin and POPs combinations on cell viability and hormone production showed synergistic, additive or antagonistic effects depending on the cell model, tested concentrations or mixture. Overall, this study provides the first comprehensive evidence that exposure to mycotoxins and/or POPs, individually or in combination, could have adverse effects on reproduction and development through multiple mechanisms.


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